Journal: Science Advances

Article Title: Targeted inhibition of hepatic de novo ceramide synthesis ameliorates MASH

doi: 10.1126/sciadv.adx2681

Figure Lengend Snippet: ( A ) Whole-body IVIS imaging of mice injected with nonlabeled LNP-siRNA (control group) or LNPs loaded Cy5-labeled siRNA. ( B ) Representative IVIS image illustrating the distribution of LNPs across various organs. ( C ) Flow cytometry analysis showing cellular uptake of Cy5-siRNA in hepatocytes. ASGR1 was used as a hepatocyte specific marker. ( D ) Distribution of Cy5-labeled siRNA among different liver cell populations in healthy mice and MASH mice fed an MCD diet for 8 weeks. Data were presented as the percentage of Cy5-positive cells within each cell type based on FACS analysis. EC, endothelial cells; HSCs, hepatic stellate cells; DCs, dendritic cells. ( E to G ) Time-course analysis of hepatic Sptlc2 mRNA levels and SPTLC2 protein levels following a single intravenous dose of LNP-siRNA (0.3 mg/kg) in MASH mice fed an MCD diet for 4 weeks. Glyceraldehyde-3-phosphate dehydrogenase was used as a loading control for Western blot analysis, and protein expression levels were normalized to that on day 2 postdosing of LNP-Scrambled siRNA (control). ( H ) Time-course analysis of hepatic Sptlc2 mRNA levels in healthy mice following siRNA treatment at 0.3 mg/kg per dose, administered either once or twice weekly. The once-weekly group received doses on days 0 and 7, while the twice-weekly group received doses on days 0, 3, 7, and 10. N = 3 mice per group. * P < 0.05, ** P < 0.01, and *** P < 0.001; one-way ANOVA.

Article Snippet: Cells were stained with an ASGR1 antibody (Proteintech, catalog no. CL488-11739, Illinois, USA) for 30 min at 4°C in the dark.

Techniques: Imaging, Injection, Control, Labeling, Flow Cytometry, Marker, Western Blot, Expressing

Journal: Frontiers in Nutrition

Article Title: Dietary Plant Lectins Appear to Be Transported from the Gut to Gain Access to and Alter Dopaminergic Neurons of Caenorhabditis elegans , a Potential Etiology of Parkinson’s Disease

doi: 10.3389/fnut.2016.00007

Figure Lengend Snippet: Lectins detected in the neurons by co-localization .

Article Snippet: Commercially available plant lectins conjugated to TRITC or rhodamine were from EY labs (San Mateo, CA, USA), Vector Labs (Burlingame, CA, USA), or Sigma-Aldrich (St. Louis, MO, USA).

Techniques:

Journal: Frontiers in Nutrition

Article Title: Dietary Plant Lectins Appear to Be Transported from the Gut to Gain Access to and Alter Dopaminergic Neurons of Caenorhabditis elegans , a Potential Etiology of Parkinson’s Disease

doi: 10.3389/fnut.2016.00007

Figure Lengend Snippet: Lectins alter number, GFP-intensity, or size of DAergic neurons without observed co-localization .

Article Snippet: Commercially available plant lectins conjugated to TRITC or rhodamine were from EY labs (San Mateo, CA, USA), Vector Labs (Burlingame, CA, USA), or Sigma-Aldrich (St. Louis, MO, USA).

Techniques: Fluorescence

Journal: bioRxiv

Article Title: Biochemical characterization of the Escherichia coli surfaceome: A focus on type I fimbriae and flagella

doi: 10.1101/2024.01.14.575581

Figure Lengend Snippet: Protein and lectin staining of released E. coli surface proteins. NT: Non-treated, AH: Acid-hydrolyzed, LF: AIEC LF82. MG: E. coli MG1655. (A) Coomassie blue stain, (B) Anti-type I fimbriae-FITC staining, (C) ConA-HRP staining.

Article Snippet: HRP-conjugated galanthus nivalis lectin (GNA) was obtained from USBiological (CliniSciences, Nanterre; G1044-25).

Techniques: Staining

Journal: bioRxiv

Article Title: Biochemical characterization of the Escherichia coli surfaceome: A focus on type I fimbriae and flagella

doi: 10.1101/2024.01.14.575581

Figure Lengend Snippet: Lectin staining of released E. coli surface proteins. (A) GNA-HRP staining. Lane 1: Mock-treated LF82; Lane 2: PNGase F-treated LF82; Lane 3: Acid hydrolyzed E. coli MG1655; Lane 4: Untreated MG1655; Lane 5: Mock-treated MG1655; Lane 6: PNGase F-treated MG1655. C: PNGase F enzyme only control; M: Molecular weight ladder. (B) WGA-biotin staining. Lane 1: LF82 non-hydrolyzed; Lane 2: LF82 acid-hydrolyzed; Lane 3: MG1655 non-hydrolyzed; Lane 4: MG1655 acid-hydrolyzed.

Article Snippet: HRP-conjugated galanthus nivalis lectin (GNA) was obtained from USBiological (CliniSciences, Nanterre; G1044-25).

Techniques: Staining, Molecular Weight

Journal: bioRxiv

Article Title: Biochemical characterization of the Escherichia coli surfaceome: A focus on type I fimbriae and flagella

doi: 10.1101/2024.01.14.575581

Figure Lengend Snippet: ConA lectin probing following PNGase or mannosidase treatment of released E. coli surface proteins. LF: AIEC LF82, MG: E. coli MG1655. NT: Non-acid hydrolyzed, AH: Acid hydrolyzed, L: Molecular weight ladder, Mock: Mock-treated sample, Man: Mannosidase-treated sample. (A) PNGase F treatment probed with ConA. (B) Anti-type I fimbria staining of membrane (A). (C) Mannosidase treatment probed with ConA.

Article Snippet: HRP-conjugated galanthus nivalis lectin (GNA) was obtained from USBiological (CliniSciences, Nanterre; G1044-25).

Techniques: Molecular Weight, Staining, Membrane